SCIENTIFIC RESEARCH

In House Research



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GULF SPECIMEN MARINE LAB
 • PO BOX 237 • PANACEA, FL 32346 USA • (850) 984-5297 • FAX (850) 984-5233






In addition to the support of offsite research and education through its biological supply program, Gulf Specimen’s staff research has led to new research models for understanding the working of neurons and contributed to the development of new diagnostic tools for understanding endotoxemia. GSML research has also explored the aquaculture potential of local seafood species and developed new markets for underutilized seafood.  Programs have focused largely on the fields of sea turtle research and aquaculture of marine species for laboratory use. They have included tag and release studies of the endangered Kemp's Ridley sea turtle (National Marine Fisheries Service); ecology and aquaculture methods for the electric ray, a biomedical model species (Howard Hughes Medical Institution); aquaculture of bryozoans as a source of anticancer drugs (National Cancer Institute); migratory patterns of blue crabs (Florida Department of Environmental Protection); ecology and pharmaceutical use of horseshoe crabs (U.S. Food and Drug Administration). A bibliography of scientific papers based on research done at Gulf Specimen Marine Laboratory is provided herein.

 Fisheries Research and Development


Gulf Specimen pioneered the development of two new commercial fisheries, rock shrimp and cannonball jellyfish.

Rock shrimp, a species new to science, was discovered in the Gulf of Mexico by the Florida Department of Natural Resources. In 1971, Jack Rudloe was the first to attempt to establish a commercial market for rock shrimp as a new seafood species, submitting samples to dealers and paying fishermen to land it for the first time. Rock shrimp is now a significant seafood product.

 
 
In 1990-92, Jack Rudloe traveled to China for the US Department of State to develop a commercial export market for American cannonball jellyfish. He researched and published techniques for the processing of jellyfish, an Asian delicacy. He also traveled to Malaysia for the same purpose. After Florida voters passed the 1996 ban on net fishing, state money was used to develop workshops based on Rudloe’s work to train dislocated commercial fishermen. Commercial jellyfish processing was done in Gulf and Duval counties in subsequent years.
 Biomedical Collaborations
       
Bryostatin - Beginning in 1968, GSML was the first supplier of marine species to the National Cancer Institute’s Natural Products Division which looks for new cancer drugs in other species. Over a period of 20 years, Gulf Specimen supplied hundreds of species for screening to NCI. Of these, bryostatin, a compound found only in the bryozoan Bugula neritina, was one of the most promising and led to extensive scientific research and publications. By 2003, bryostatin had received clinical approval for the treatment of esophageal cancer and was in the final stages for approval as a breast cancer drug.

Watch Anne's cancer video:  Anne Rudloe Speaks
 Electrical Ray Neurobiology Research - In the mid 1980s, the Howard Hughes Medical Institute contracted with Gulf Specimen Marine Laboratory (then called the Panacea Institute of Marine Science) to provide lesser electric rays, Narcine brasiliensis, for neurophysiological research into the biochemistry of the neurotransmitter acetylcholine. Since nothing was known of the biology or distribution of the species, GSML did extensive survey, ecological and behavioral research in conjunction with the project. In 2003, federally funded research began at GSML on technological applications of biomagnetism in Narcine.
 Limulus Lysate - Field studies funded by the US Food and Drug Administration measured the impact of biomedical blood collection on long term survival of horseshoe crabs. The results were used to develop conservation protocols to insure that biomedical use would not reduce the populations of this species.

Research Publications of Gulf Specimen Marine Laboratory
Hsieh, Y-H.P. F-M Leong & J. Rudloe. 2001. Jellyfish as Food. Hydrobiological, Klaver Academic Publishers. 451:11-17

Rudloe, A. 2000. The ecological importance of fresh water input to coastal Florida ecosystems. Woodville Karst Plain Symposium, October 9, 1998. Florida Department of Environmental Protection.

Rudloe, A. 1999. Behavioral Adaptations to Environmental Change. In: Tested Studies for Laboratory Teaching. Vol. 20. S. J. Karecher, ed. Proc. 20th Workshop Conference of the Assoc. for Biology Laboratory Education.

Rudloe, A.1995. Characterization of an inshore population of the Kemp's ridley sea turtle in the northeastern Gulf of Mexico. In: Proc. 12th Annual Workshop on Sea Turtle Biology and Conservation. February 25-28, 1992, Jekyll Island, Georgia.

Hsieh, Y-H.P. and J. Rudloe. 1994 Potential of Utilizing Jellyfish as Food in Western Countries, Trends in Food Science & Technology, July, Vol.5: 225-229

Pettit, G.R., F. Gao, D. Sengupta, J.C. Coll, C.L. Herald, D.L. Doubek, J.M. Schmidt, J.R. Van Camp, J.J. Rudloe, and R.A. Nieman. 1991. Isolation and Structure of Bryostatins 14 and 15. Tetrahedron January, Vol 47, No.22 pp 3601-3620.

Rudloe, A., J. Rudloe and L.H. Ogren. 1991 Occurrence of immature Kemp's ridley turtles, Lepidochelys kempi in coastal waters of northwest Florida. Northeast Gulf Sci. 12(1):49-53.

Rudloe, A. 1989. Captive maintenance of the lesser electric ray, Narcine brasiliensis, with observations of feeding behavior. Prog. Fish Culturalist 51: 37-41.

Rudloe, A.1989 Habitat preferences, movement, size frequency patterns and reproductive seasonality of the lesser electric ray, Narcine brasiliensis. Northeast Gulf Science 10:103-112.

Rudloe, A.1985 Variation in the expression of lunar and tidal behavioral rhythms in the horseshoe crab, Limulus polyphemus. Bull. Mar. Sci. 36: 388-395.

Rudloe, A. 1983 Preliminary studies of the mariculture potential of the slipper lobster, Scyllarides nodifer.Aquaculture 34: 165-169.

Pettit, G. R., Y. Kamano, R. Aoyagi, C.L. Herald, DlL. Douber, J.M. Schmidt and J.J. Rudloe. September. 1983. Antineoplastic Agents 100, The Marine Bryozoan Amathia convoluta. Tetrahedron, Vol.41, No.6. pp .985-994

Rudloe, A. 1983 The effect of heavy bleeding on mortality of the horseshoe crab, Limulus polyphemus, in the natural environment. J. Invert. Path 42: 167-176.

Rudloe, A. 1982 Man's Influence as an Environmental Threat to Limulus Physiol. Biol. of Horseshoe Crabs:   Normal and Envir. Stressed Animals.  Alan R. Liss, Publ., pp. 297-300.

Rudloe, A.1981 Aspects of the biology of juvenile horseshoe crabs, Limulus polyphemus. Bull. Mar. Sci., Vol. 32: 125-133.

Rudloe, A.1980 Orientation by horseshoe crabs, Limulus polyphemus, in a Wave Tank. Mar. Beh. Physio., 7: 199‑211.

Rudloe, A.1980 The breeding behavior and patterns of movement of Horseshoe Crabs, Limulus polyphemus, in the vicinity of breeding beaches in Apalachee Bay, Fl.  Est. 3:177-183.

Rudloe, A.1979.Locomotor and light responses of larvae of the horseshoe crab, Limulus polyphemus. Biological Bulletin 157: 494-501.

Rudloe, A.1979 Limulus polyphemus:  a review of the ecologically significant literature.  In Cohen, E. (ed.) "Biomedical Applications of the Horseshoe Crab (Limulidae)."  Alan R. Liss, Publ., New York, 27-35.

Rudloe, Anne and W.F. Herrnkind. 1976.  Orientation of Limulus polyphemus in the vicinity of breeding beaches. Mar. Beh. Physiol 4:75-89.

Kritzler, H. and A. Eidemiller.1972. A diver monitored dredge for sampling motile epibenthos. J. Mar. Biol. Assoc. U.K. 52:553-556.

Eidemiller, A.1969. Entry behavior of the pea crab, Pinnotheres maculatus Say. Quart. J. Florida Aca. Sci. 32:266-274.

Reports to Government Agencies
1996. Rudloe, J. Methods to Harvest and Process the Cannonball Jellyfish, Stomolophus meleagris, A Training Manual.Florida Dept of Agriculture;70 pages.

1995. Rudloe, J. Potential for New Initiatives in Fisheries and Marine-Aquaculture possibilities in the Florida Panhandle. A Report to the Bureau of Vocational Programs and Services Division of Applied Technology and Adult Education Florida Education Center.

1995. Rudloe, J and R.P. Jones, The Harvesting Processing of Florida Cannonball Jellyfish. Report to the Florida International Affairs Commission, Florida Department of Commerce, Southeastern Fisheries

1995. Rudloe, J. The Jellyfish Fishery in China. A Report to NOAA International Cooperation and Development Research and Scientific Exchanges Division. 29 pgs, illustrations. February 15, 1995

1995. Rudloe, J. Madei and A. Rudloe. Report to Congress on the Aquaculture of Endangered and Threatened Species and Restoration of Aquatic Systems, Office of Technology Assessment.

Rudloe, J. "Use of the Malaysian Dry Salting Technique for Processing Cannonball Jellyfish. Gulf Specimen Press, for Florida Department of Agriculture, Seafood and Aquaculture Marketing. 41 Pages.

1992. Rudloe, J. Jellyfish: A New Fishery for the Florida Panhandle. Apalachee Regional Planning Council, Economic Development Authority, Pp 1-35
Marine Biology Articles
How Male Blue Crabs Attract Females

Context Dependent Courtship Display by Male Blue Crabs

Crabbing in the Chesapeake